Can we use a debit MasterCard when picking up our vehicle if we have already prepaid for the vehicle online

So it can or cannot be used?

Yes but your deposit will be ~$250 that will be held against your debit card and you will not have access to the money until the car is returned.

Nope... Only cridet cards, and the amount will not be charged, it will be held, until you return the car thats when you can tell them to deduct from your debt card, but do tell this to them when you pick it up and when you drop it off.