Is that pet deposit a one time fee?

Yes

My brother has a turtle and a fish tank would he pay the fee? And what else would be required

It it a one time thing they also work with you if you can't fund the hole thing at once they add so much to your bill I think it 250 deposit and the animal has to be spaded or nuder and has to be a small pet Also Has To Have All Its Shots By A Vet Is What They Told Me