1.) Do they take Mastercard (Visa) debit cards at Universal Studios Florida? 2.) Does it cost anything to pick up the tickets at the Will Call Kisok?

Yes they take Mastercard and no it doesn't cost anything to pick up tickets at will call kisok

Yes they take all cards. Also I recommend just either printing those tickets or using the free app for your tickets. You will likely waste 1 hour in line at the kiosks or booths to pick up tickets.

Yes and no!

Ofcourse