If you plan to get off at a stop to go exploring and wait for the next bus you can try find a bathroom there. The longest stop however (other than the first/last) is golden gate bridge and there should be enough time to run to the bathroom there. Often the guides use this as their bathroom break. All other stops are for as long as it takes for people to get on or off the bus.
I had no problem since you can get off at any of theirs stops n find a bathroom. Love tbeir itinerary. Must do Sausolito.
No, it's very much difficult
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